Key Features Specially formulated Single-Use Arachidonic Acid (peroxide free) for use in cell culture or other in vitro experiments Peroxide free and sealed under argon to maintain product integrity Each pack contains 10 single-use ampules (>1 ml fatty acid at 500 µg/ml in ethanol) Single-use arachidonic acid (peroxide free) consists of 10 sealed ampules, each containing >1 ml of ethanolic solution of fatty acid at a concentration of 500 µg/ml. Peroxides were removed by silicic acid chromatography. The headspace in each ampule is purged with argon. Single-use polyunsaturated fatty acids (PUFAs) are intended for analytical and research applications where absence of oxidized adducts is desired. Ampules have been optimized for one-time use. For best results, use promptly after opening. Arachidonic acid (Item No. 90010) is a substrate for the biosynthesis of eicosanoid second messengers. PUFAs, including arachidonic acid, oxidize spontaneously on exposure to room air, yielding a complex mixture of fatty acid peroxides. Oxidation of PUFAs can be particularly problematic in samples stored in capped vials for prolonged periods, even at low temperatures. These single-use ampules are designed to circumvent this issue.
Available on backorder
An ethanolic solution in sealed ampules with the headspace purged with argon
Formal name: 5Z,8Z,11Z,14Z-eicosatetraenoic acid
Synonyms: Single-Use AA (peroxide free)
Molecular weight: 304.5
CAS: 506-32-1
Purity: ≥98%
Formulation: A solution in ethanol
Application|Mass Spectrometry||Product Type|Biochemicals|Lipids|Fatty Acids||Research Area|Lipid Biochemistry|Fatty Acids
The maintenance of embryonic stem cells in culture typically requires feeder cells and various exogenous factors found in serum. Murine embryonic stem (mES) cells can be maintained in the absence of feeder cells and serum but require the leukemia inhibitor factor (LIF) and bone morphogenic protein (BMP) to prevent differentiation and promote self-renewal. SC-1 is a small molecule activator of stem cell renewal that allows the propagation of OG2 mES cells for at least 10 passages in an undifferentiated state.{14533} The activity of SC-1 is mediated by the combined inhibition of RasGAP and ERK1 with Kd values of 98 and 212 nM, respectively.{14533} Inhibition of RasGAP increases Ras signaling via the PI3-kinase pathway which promotes self-renewal, whereas inhibition of ERK blocks differentiation.
Available on backorder
1,2-Dipalmitoyl-sn-glycero-3-PC (DPPC) is a zwitterionic glycerophospholipid commonly used in the formation of lipid monolayers, bilayers, and liposomes for use in a variety of applications.{14480,24261,14478,14479} It has been used in the formation of proteoliposomes for implantation of γ-glutamyl transpeptidase into human erythrocyte membranes.{14478} Incorporation of glycosphingolipid antigens into DPPC-containing liposomes increases the immunogenicity of the antigens in mice.{14479}
Available on backorder
The PtdIn phosphates play an important role in the generation and transduction of intracellular signals.{8344,4096,14518} PtdIns-(3,4,5)-P3-biotin is an affinity probe which allows the PIP3 to be detected through an interaction with the biotin ligand. This design allows PtdIns-(3,4,5)-P3-biotin to serve as a general probe for any protein with a high affinity binding interaction with inositol-(3,4,5)-triphosphate phospholipids, such as phosphatidylinositol 3-kinase, PTEN, or PH-domain-containing proteins.
Available on backorder
The maintenance of embryonic stem cells in culture typically requires feeder cells and various exogenous factors found in serum. Murine embryonic stem (mES) cells can be maintained in the absence of feeder cells and serum but require the leukemia inhibitor factor (LIF) and bone morphogenic protein (BMP) to prevent differentiation and promote self-renewal. SC-1 is a small molecule activator of stem cell renewal that allows the propagation of OG2 mES cells for at least 10 passages in an undifferentiated state.{14533} The activity of SC-1 is mediated by the combined inhibition of RasGAP and ERK1 with Kd values of 98 and 212 nM, respectively.{14533} Inhibition of RasGAP increases Ras signaling via the PI3-kinase pathway which promotes self-renewal, whereas inhibition of ERK blocks differentiation.
Available on backorder