A fluorogenic probe that acts as a substrate for specific cytochrome P450 (CYP) isoforms

Dibenzylfluorescein – 10 mg

Brand:
Cayman
CAS:
97744-44-0
Storage:
-20
UN-No:
Non-Hazardous - /

Dibenzylfluorescein is a fluorogenic probe that acts as a substrate for specific cytochrome P450 (CYP) isoforms, including CYP3A4, CYP2C8, CYP2C9, CYP2C19, and aromatase (CYP19).{27561,27560,27562} Dibenzylfluorescein is dealkylated by these CYP isoforms to produce fluorescein benzyl ether, which is further hydrolyzed to fluorescein by the addition of base (typically 2 M NaOH).{27562} Dibenzylfluorescein is typically used near its apparent Km value of 0.87-1.9 µM.{27561,27560,27562} The fluorescence of fluorescein is evaluated using excitation/emission wavelengths of 485/538 nm. Dibenzylfluorescein is used to detect changes in CYP catalytic activity caused by drugs or disease.{27561,27563}  

 

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Description

A fluorogenic probe that acts as a substrate for specific cytochrome P450 (CYP) isoforms, including CYP3A4, CYP2C8, CYP2C9, CYP2C19, and aromatase (CYP19); used to detect changes in CYP catalytic activity caused by drugs or disease

Formal name: 2-[3-oxo-6-(phenylmethoxy)-3H-xanthen-9-yl]-benzoic acid, phenylmethyl ester

Synonyms:  DBF|NSC 645658

Molecular weight: 512.6

CAS: 97744-44-0

Purity: ≥98%

Formulation: A crystalline solid

Application|Enzyme Activity||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Enzyme Substrates||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Toxicology|Drug Metabolism|Cytochrome P450

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