A chromophoric substrate that is used to quantify inorganic phosphate (Pi) in the presence of purine nucleoside phosphorylase

7-methyl-6-Thioguanosine (technical grade) – 5 mg

Brand:
Cayman
CAS:
55727-10-1
Storage:
-20
UN-No:
Non-Hazardous - /

7-methyl-6-Thioguanosine is a chromophoric substrate that is used to quantify inorganic phosphate (Pi). In the presence of purine nucleoside phosphorylase, it reacts with Pi to produce 7-methyl-6-thioguanine and ribose 1-phosphate, with the increase in 7-methyl-6-thioguanine followed by continuous spectrophotometry at an absorbance of 355-360 nm.{26928} 7-methyl-6-Thioguanosine is also used to measure changes in Pi levels in coupled enzyme systems, including assays of the activities of protein phosphatases, phosphorylase kinases, GTPases, and ATPases.{26928,26932,26930,26931}  

 

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Description

A chromophoric substrate that is used to quantify inorganic phosphate (Pi) in the presence of purine nucleoside phosphorylase, followed by continuous spectrophotometry at an absorbance of 355-360 nm; used to measure changes in Pi levels in coupled enzyme systems

Formal name: 2-amino-6,9-dihydro-7-methyl-9-β-D-ribofuranosyl-6-thioxo-1H-purinium, inner salt

Synonyms:  MESG

Molecular weight: 313.3

CAS: 55727-10-1

Purity: ≥85%

Formulation: A crystalline solid

Product Type|Biochemicals|Labeling & Detection|Enzyme Substrates||Research Area|Cell Biology|Cell Signaling|GTPases||Research Area|Epigenetics, Transcription, & Translation|RNA, DNA, & Protein Synthesis

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