A light-emitting biomolecule that serves as a substrate for luciferases or constituent of photoproteins;

Coelenterazine – 5 mg

Brand:
Cayman
CAS:
55779-48-1
Storage:
-20
UN-No:
Non-Hazardous - /

Coelenterazine is a luciferin, a light-emitting biomolecule that serves as a substrate for luciferases or as a constituent of photoproteins, including aequorin.{22804} The oxidation of coelenterazine to coelenteramide is accompanied by emission of blue light (emission maximum, 460-470 nm). Luciferase-mediated oxidation of coelenterazine or a derivative is used as an energy donor, typically to a form of green or yellow fluorescent protein, in bioluminescent resonance energy transfer studies.{26296,26290} Alternatively, the calcium-mediated release of coelenterazine from aequorin, followed by non-enzymatic oxidation of this compound, results in bioluminescence. As light emission depends on both calcium and cellular redox status, this reaction is used to non-fluorescently detect changes in calcium level and redox status.{5885,6107}  

 

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Description

A light-emitting biomolecule that serves as a substrate for luciferases or constituent of photoproteins;,oxidation to coelenteramide is accompanied by emission of blue light (emission maximum = 460-470 nm); used in BRET studies and to detect changes in calcium level and redox status

Formal name: 6-(4-hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]-8-(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one

Synonyms:  Luciferin (Oplophorus)|Preluciferin (Watasenia)

Molecular weight: 423.5

CAS: 55779-48-1

Purity: ≥85%

Formulation: A crystalline solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology|Cell Signaling|Calcium Mobilization

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