A fluorogenic substrate for

7-Ethoxyresorufin – 1 mg

Brand:
Cayman
CAS:
5725-91-7
Storage:
-20
UN-No:
Non-Hazardous - /

7-Ethoxyresorufin (7-ER) is a fluorogenic substrate for, and competitive inhibitor of, the cytochrome P450 (CYP) isoform CYP1A1 (IC50 = 0.1 µM).{26089,26092,26091} Upon enzymatic cleavage by CYP1A1, resorufin is released and its fluorescence can be used to quantify CYP1A1 activity. Resorufin displays excitation/emission maxima of 570/580 nm, respectively.{50356} 7-ER has been used in the study of arachidonic acid (Item Nos. 90010 | 90010.1 | 10006607) metabolism and the mechanisms of vasodilation.{15972,12467} 7-ER is a noncompetitive inhibitor of neuronal nitric oxide synthase (nNOS; Ki = 0.76 µM).{26088,26090}  

 

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Description

A fluorogenic substrate for, and inhibitor of, CYP1A1; upon enzymatic cleavage by CYP1A1, resorufin is released and its fluorescence can be used to quantify CYP1A1 activity; ex/em = 570/580 nm, respectively; a noncompetitive inhibitor of nNOS (Ki = 0.76 µM)

Formal name: 7-ethoxy-3H-phenoxazin-3-one

Synonyms:  7-ER|7-Ethoxyphenoxazone|Resorufin ethyl ether

Molecular weight: 241.2

CAS: 5725-91-7

Purity: ≥95%

Formulation: A solid

Application|Enzyme Activity||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Enzyme Substrates||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Product Type|Biochemicals|Small Molecule Inhibitors|Cytochrome P450||Product Type|Biochemicals|Small Molecule Inhibitors|Nitric Oxide Synthases||Research Area|Lipid Biochemistry||Research Area|Oxidative Stress & Reactive Species|Reactive Nitrogen|nNOS||Research Area|Toxicology|Drug Metabolism|Cytochrome P450

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