A redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms; readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent

5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) – 25 mg

Brand:
Cayman
CAS:
90217-02-0
Storage:
-20
UN-No:
Non-Hazardous - /

5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms.{25823,25821,25822} This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm).{25822,25820} While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm; emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter.{25823,25821} For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable.  

 

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Description

A redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms; readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm)

Formal name: 5-cyano-2,3-bis(4-methylphenyl)-2H-tetrazolium, monochloride

Synonyms:  CTC|Cyanotolyl Tetrazolium Chloride

Molecular weight: 311.8

CAS: 90217-02-0

Purity:

Formulation: A crystalline solid

Application|Flow Cytometry||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area

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