Several cyclic nucleotide phosphodiesterases (PDE2, -5, -6, -10, and -11) contain a tandem of GAF domains in their N-terminal regulatory region that are thought to mediate cAMP- or cGMP-induced stimulation of adenylyl and guanylyl cyclases.{24263} Sp-cyclic AMPS (Sp-cAMPS) is a derivative of cAMP that binds the PDE10 GAF domain (EC50 = 40 μM).{24263} It has been used to study GAF-mediated stimulation of PDE10.{24263}
A derivative of cAMP that binds the PDE10 GAF domain (EC50 = 40 μM) and has been used to study GAF-mediated stimulation of PDE10
Formal name: cyclic 3′,5′-[hydrogen (S)-phosphorothioate] adenosine, monosodium salt
Synonyms: Sp-cAMPS
Molecular weight: 367.3
CAS: 142439-95-0
Purity: ≥98%
Formulation: A crystalline solid
Product Type|Biochemicals||Research Area|Cell Biology|Cell Signaling|cAMP Signaling
The tetracycline repressor (TetR) is a transcriptional regulator which normally binds tightly to its palindromic tetO operator DNA, blocking gene expression.{17508} Tet causes the repressor to dissociate from the DNA, allowing transcription to occur. A novel reverse TetR (revTetR) requires tetracycline as a co-repressor to bind tetO and block transcription.{17509,17510} Anhydrotetracycline (hydrochloride) is a powerful effector in both the tetracycline repressor (TetR) and reverse TetR (revTetR) systems, binding the Tet repressor 35-fold more strongly than Tet.{17508,17511} Moreover, anhydrotetracycline poorly binds the 30S ribosomal subunit, compared to Tet,{17512} so it does not act as a general inhibitor of translation and is a poor antibiotic. Perhaps related to this, the concentration of anhydrotetracycline that inhibits eukaryotic cell growth is more than a 1,000-fold above the dose that alters transcription through TetR.{17508}
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