A steroidal lactone with diverse biological activities; binds to and induces aggregation of vimentin intermediate filaments in cultured endothelial cells and fibroblasts

Withaferin A – 1 mg

Brand:
Cayman
CAS:
5119-48-2
Storage:
-20
UN-No:
Non-Hazardous - /

Withaferin A is a steroidal lactone that has been found in W. somnifera with diverse biological activities.{21343,21342} It binds to and induces aggregation of vimentin intermediate filaments in cultured endothelial cells and fibroblasts, inducing apoptosis when used at concentrations ranging from 2 to 25 μM. It also induces formation of perinuclear aggregates in other intermediate filament networks including peripherin, neurofilament-triplet protein, and keratin as well as disrupts the organization of microtubules and actin/microfilaments.{21342} Withaferin A induces noncanonical ferroptosis via induction of lipid peroxidation through interaction with Kelch-like ECH-associated protein 1 (KEAP1) in IMR-32 neuroblastoma cells, an effect that is blocked by the ferroptosis inhibitors ciclopirox olamine and ferrostatin-1 (Item No. 17729).{35972} It also binds to and inhibits glutathione peroxidase 4 (GPX4) in IMR-32 cells. In vivo, withaferin A (4 mg/kg) reduces intratumor GPX4 expression and induces tumor regression in an IMR-32 mouse xenograft model. Withaferin A (2 mg/kg) also inhibits angiogenesis in a mouse model of injury-induced corneal neovascularization.{21343}  

 

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Description

A steroidal lactone with diverse biological activities; binds to and induces aggregation of vimentin intermediate filaments in cultured endothelial cells and fibroblasts, inducing apoptosis from 2-25 μM; induces formation of perinuclear aggregates in a variety of other intermediate filament networks; induces noncanonical ferroptosis via induction of lipid peroxidation through interaction with KEAP1 in IMR-32 neuroblastoma cells; binds to and inhibits GPX4 in IMR-32 cells; reduces intratumor GPX4 expression and induces tumor regression in an IMR-32 mouse xenograft model at 4 mg/kg; inhibits angiogenesis in a mouse model of injury-induced corneal neovascularization at 2 mg/kg

Formal name: 5β,6β-epoxy-4β,22R,27-trihydroxy-1-oxo-δ-lactone-ergosta-2,24-dien-26-oic acid

Synonyms:  NSC 101088|NSC 273757

Molecular weight: 470.6

CAS: 5119-48-2

Purity: ≥95%

Formulation: A crystalline solid

Product Type|Biochemicals|Microtubule Dynamics||Product Type|Biochemicals|Natural Products||Research Area|Cancer|Cell Death||Research Area|Cell Biology|Cell Death|Apoptosis||Research Area|Cell Biology|Cell Death|Ferroptosis||Research Area|Cell Biology|Cytoskeleton & Motor Proteins

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