A SAHA derivative with a benzophenone crosslinker and an alkyne tag to be used for profiling HDAC activities in proteomes and live cells; labels HDAC complex proteins both in proteomes at 100 nM and in live cells at 500 nM; IC50 = ~3 μM for inhibition of HDAC activity in HeLa cell nuclear lysates in an HDAC activity assay

SAHA-BPyne – 1 mg

Brand:
Cayman
CAS:
930772-88-6
Storage:
-20
UN-No:
Excepted Quantity - 1230 / 3|6.1

Suberoylanilide hydroxamic acid (SAHA) is a class I and class II histone deacetylase (HDAC) inhibitor that binds directly to the catalytic site of the enzyme thereby blocking substrate access.{14739} SAHA-BPyne is a SAHA derivative with a benzophenone crosslinker and an alkyne tag intended to be used for profiling HDAC activities in proteomes and live cells.{15936,19509} Such terminal alkyne groups can be used in linking reactions, known as click chemistry, characterized by high dependability and specificity of azide-alkyne bioconjugation reactions.{17991,17992} SAHA-BPyne labels HDAC complex proteins both in proteomes at 100 nM and in live cells at 500 nM and demonstrates an IC50 value of ~3 μM for inhibition of HDAC activity in HeLa cell nuclear lysates in an HDAC activity assay.{15936}  

 

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SKU: 10675 - 1 mg Category:

Description

A SAHA derivative with a benzophenone crosslinker and an alkyne tag to be used for profiling HDAC activities in proteomes and live cells; labels HDAC complex proteins both in proteomes at 100 nM and in live cells at 500 nM; IC50 = ~3 μM for inhibition of HDAC activity in HeLa cell nuclear lysates in an HDAC activity assay

Formal name: N1-hydroxy-N8-[4-[4-[(1-oxo-5-hexyn-1-yl)amino]benzoyl]phenyl]-octanediamide

Synonyms:  Click Tag™ SAHA-BPyne|Suberoylanilide Hydroxamic Acid-BPyne

Molecular weight: 477.6

CAS: 930772-88-6

Purity: ≥98%

Formulation: A solution in methanol

Application|Click Chemistry||Product Type|Biochemicals|Labeling & Detection|Reactive Probes||Product Type|Biochemicals|Small Molecule Inhibitors|Deacetylases||Research Area|Epigenetics, Transcription, & Translation|Erasers|Histone Deacetylation

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