Description
ApNA is one of several nitroaniline fatty acid amides which can be used to measure FAAH activity.{8928} FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its nominal endogenous substrate AEA (Item No. 90050). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. (See also Decanoyl p-Nitroaniline – Catalog No. 90349). Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer.
Formal name: N-(4-nitrophenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide
Synonyms: ApNA
Molecular weight: 424.6
CAS: 119520-58-0
Purity: ≥98%
Formulation: A solution in methyl acetate
Application|Enzyme Activity||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Enzyme Substrates||Product Type|Biochemicals|Lipids|Fatty Acid Conjugates||Product Type|Biochemicals|Small Molecule Inhibitors|Fatty Acid Metabolism||Research Area|Lipid Biochemistry|Endocannabinoid/Endocannabinoid-like||Research Area|Neuroscience|Cannabinoid Research|Endocannabinoids
