A biotinylated reagent used for the detection and quantification of apurinic/apyrimidinic (AP) sites in damaged DNA; reacts with aldehyde groups formed when reactive oxygen species depurinate DNA

Aldehyde Reactive Probe (trifluoroacetate salt) – 5 mg

Brand:
Cayman
CAS:
627090-10-2
Storage:
-20
UN-No:
Non-Hazardous - /

DNA is continually damaged by endogenous and environmental agents leading to the formation of abasic (apurinic/apyrimidinic, AP) sites that are disruptive to DNA synthesis. Aldehyde Reactive Probe (ARP) is a biotinylated reagent for the detection and quantification of AP sites in damaged DNA. ARP reacts with aldehyde groups formed when reactive oxygen species depurinate DNA, resulting in covalent linkage of biotin to these AP sites.{16234} The biotin-tagged DNA can then be detected using common avidin-conjugated reporters such as avidin-HRP. The ARP method is highly sensitive, enabling detection of 2.4 AP sites per 1×107 nucleotides of DNA.{16233}  

 

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SKU: 10009350 - 5 mg Category:

Description

A biotinylated reagent used for the detection and quantification of apurinic/apyrimidinic (AP) sites in damaged DNA; reacts with aldehyde groups formed when reactive oxygen species depurinate DNA, thereby covalently linking biotin to these AP sites.

Formal name: (6aR)-hexahydro-2-oxo-2-[(aminooxy)acetyl]hydrazide,1H-thieno[3aS,4S-d]imidazole-4-pentanoic acid, trifluoroacetate salt

Synonyms:  ARP|O-(Biotinylcarbazoylmethyl) Hydroxylamine

Molecular weight: 445.4

CAS: 627090-10-2

Purity: ≥95%

Formulation: A crystalline solid

Product Type|Biochemicals|Labeling & Detection|Reactive Probes||Research Area|Oxidative Stress & Reactive Species|DNA/RNA Oxidative Damage

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